Hotel Dieu

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

Ulrich Jarry^*1,2, Noémie Joalland^*1,2, Cynthia Chauvin^*1,2, Béatrice Clemenceau^1,2,3, Claire Pecqueur^1,2, Emmanuel Scotet^1,2

¹INSERM, CNRS, Université d'Angers, Université de Nantes, CRCINA, ²Immunotherapy, Graft, Oncology, LabEx IGO, ³Hopital de Nantes, Hotel Dieu

Here, we present a protocol for the preparation and the stereotaxic administration of allogeneic human lymphocytes in immunodeficient mice carrying orthotopic human primary brain tumors. This study provides a proof-of-concept for both the feasibility and the antitumor efficacy of intrabrain-delivered cellular immunotherapies.