Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils FormationGiulia Panattoni 1,2, Lucia Rota 1, Emanuela Colla 1
1Bio@SNS Laboratory, Scuola Normale Superiore, 2International School for Advanced Studies (SISSA/ISAS)
The goal of this protocol is to provide a cell-based system that replicates the formation of alpha-synuclein aggregates in vivo. Intracellular alpha-synuclein inclusions are seeded in primary neurons by the internalization and propagation of exogenous administered native microsomes-associated alpha-synuclein aggregates isolated from diseased alpha-synuclein transgenic mice.
