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Université Lyon

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
Céline Delloye-Bourgeois *1, Arnaud Jacquier *1, Julien Falk 1, Valérie Castellani 1
1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534, University of Lyon

We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

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Cancer Research

Evaluation of the Feasibility, Safety, and Accuracy of an Intraoperative High-intensity Focused Ultrasound Device for Treating Liver Metastases
Aurélien Dupre 1,2, David Melodelima 2, David Perol 3, Yao Chen 1, Jérémy Vincenot 2, Jean-Yves Chapelon 2, Michel Rivoire 1,2
1Department of Surgical Oncology, Centre Léon Bérard, 2Applications des ultrasons à la thérapie (LabTAU), Institut national de la santé et de la recherche médicale (INSERM), Centre Léon Bérard, Université Lyon 1, Université Lyon, 3Biostatistics and Treatment Evaluation Unit, Centre Léon Bérard

Here, we present an ablate-and-resect prospective study to evaluate the feasibility, safety, and accuracy of intraoperative high-intensity focused ultrasound ablation in patients undergoing hepatectomy for liver metastases.

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Neuroscience

Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons
Arnaud Jacquier 1,2, Valérie Risson 1, Laurent Schaeffer 1,3
1Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217, Université Lyon, 2Unité Fonctionnelle de Neurogénétique Moléculaire, Hospices Civils de Lyon, 3Centre de Biotechnologie Cellulaire, Hospices Civils de Lyon

The goal of this technique is to prepare a highly enriched culture of primary motoneurons (MNs) from murine spinal cord. To evaluate the consequences of mutations causing MN diseases, we describe here the isolation of these isolated MNs and their transfection by magnetofection.

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