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First Affiliated Hospital of Jinan University

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
Ming Fu 1, Zefeng Lin 2, Huiting Lin 2, Yanlu Tong 2, Hezhen Wang 2, Hongjiao Chen 2, Yan Chen 2, Ruizhong Zhang 2
1First Affiliated Hospital of Jinan University, 2Department of Pediatric Surgery, Guangzhou Women & Children's Medical Center, Guangzhou Medical University

This article describes in detail a method based on silver nanoparticles for ameliorating biliary atresia syndrome in an experimental biliary atresia mouse model. A solid understanding of the reagent preparation process and the neonatal mouse injection technique will help familiarize researchers with the method used in neonatal mouse model studies.

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Bioengineering

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
Yingwei Wang *1,2, Cheng Lu *1,2, Chengzhi He 3, Baoxin Chen 1,2, Youling Zheng 1,2, Junming Zheng 1,2, Jianhua Zhang 4, Zheng Wu 1,2
1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, 2Department of Developmental and Regenerative Biology, Jinan University, 3Nansha College Preparatory Academy, 4Department of Cardiology, First Affiliated Hospital of Jinan University

This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property.

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Medicine

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis
Yan Tian *1, Junyu Huang *1, Ming Fu 1, Qiuming He 1, Jiale Chen 1, Yan Chen 1, Ruizhong Zhang 1, Wei Zhong 1
1Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University

Necrotizing enterocolitis (NEC) is the most severe gastrointestinal (GI) disease that often occurs in premature infants, especially very low birth weight infants, with high mortality and unclear pathogenesis. The cause of NEC may be related to inflammatory immune regulatory system abnormalities. An NEC animal model is an indispensable tool for NEC disease immune research. NEC animal models usually use C57BL/6J neonatal mice; BALB/c neonatal mice are rarely used. Related studies have shown that when mice are infected, Th2 cell differentiation is predominant in BALB/c mice compared to C57BL/6J mice. Studies have suggested that the occurrence and development of NEC are associated with an increase in T helper type 2 (Th2) cells and are generally accompanied by infection. Therefore, this study used neonatal BALB/c mice to induce an NEC model with similar clinical characteristics and intestinal pathological changes as those observed in children with NEC. Further study is warranted to determine whether this animal model could be used to study Th2 cell responses in NEC.

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Neuroscience

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
Qiwei Fan *1, Xiaotan Zhang *2, Yuan Wang 3, Xiaoyu Wang 1
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University

Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit assembly.

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