Instituto Tecnológico de Morelia

1 ARTICLES PUBLISHED IN JoVE

Biology

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Ivanna Karina Olivares-Marin¹, Juan Carlos González-Hernández², Carlos Regalado-Gonzalez¹, Luis Alberto Madrigal-Perez³

¹Department of Chemistry, Universidad Autónoma de Querétaro, ²Department of Biochemical Engineering, Instituto Tecnológico de Morelia, ³Department of Biochemical Engineering, Instituto Tecnológico Superior de Ciudad Hidalgo

Here we present a protocol to estimate the respiratory and fermentative metabolism by fitting the exponential growth of Saccharomyces cerevisiae to the exponential growth equation. Calculation of the kinetic parameters allows for the screening of influences of substances/compounds on fermentation or mitochondrial respiration.