Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Na Tang^1,2, Yaoyao Zhang¹, Miriam Pedrera¹, Pengxiang Chang¹, Susan Baigent¹, Katy Moffat¹, Zhiqiang Shen², Venugopal Nair¹, Yongxiu Yao¹

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, ²Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.