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Penn State College of Medicine

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
Alex Y. Lin 1,2, Yifu Ding 1,2,3, Daniel J. Vanselow 1,2, Spencer R. Katz 1,2,3, Maksim A. Yakovlev 1,2, Darin P. Clark 4, David Mandrell 5, Jean E. Copper 1,2, Damian B. van Rossum 1,2, Keith C. Cheng 1,2
1The Jake Gittlen Laboratories for Cancer Research, Penn State College of Medicine, 2Division of Experimental Pathology, Department of Pathology, Penn State College of Medicine, 3Medical Scientist Training Program, Penn State College of Medicine, 4Center for In Vivo Microscopy, Duke University Medical Center, 5KTM Research

We developed protocols and designed a custom apparatus to enable embedding of millimeter-scale specimens. We present sample preparation procedures with an emphasis on embedding in acrylic resin and polyimide tubing to achieve rigid immobilization and long-term storage of specimens for the interrogation of tissue architecture and cell morphology by micro-CT.

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Immunology and Infection

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis
Shirley Albano-Aluquin 1, Jozef Malysz 2, Michal Kidacki 3, Manohar Ratnam 4, Nancy J. Olsen 1
1Department of Medicine, Penn State University, 2Department of Pathology, Penn State University, 3Penn State College of Medicine, 4Department of Oncology, Wayne State University School of Medicine

The protocol illustrates the use of histopathologic examination and immunohistochemistry to profile the folate receptor beta macrophage and its relationship with the total immune cell infiltrate in temporal artery biopsies in giant cell arteritis.

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Biology

Electroporation of Plasmid DNA into Mouse Skeletal Muscle
Brian A. Hain 1, David L. Waning 1
1Department of Cellular and Molecular Physiology, Penn State College of Medicine

Electroporation of plasmid DNA into skeletal muscle is a viable method to modulate gene expression without compromising muscle contractility in mice.

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