Penn State College of Medicine

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Alex Y. Lin^1,2, Yifu Ding^1,2,3, Daniel J. Vanselow^1,2, Spencer R. Katz^1,2,3, Maksim A. Yakovlev^1,2, Darin P. Clark⁴, David Mandrell⁵, Jean E. Copper^1,2, Damian B. van Rossum^1,2, Keith C. Cheng^1,2

¹The Jake Gittlen Laboratories for Cancer Research, Penn State College of Medicine, ²Division of Experimental Pathology, Department of Pathology, Penn State College of Medicine, ³Medical Scientist Training Program, Penn State College of Medicine, ⁴Center for In Vivo Microscopy, Duke University Medical Center, ⁵KTM Research

We developed protocols and designed a custom apparatus to enable embedding of millimeter-scale specimens. We present sample preparation procedures with an emphasis on embedding in acrylic resin and polyimide tubing to achieve rigid immobilization and long-term storage of specimens for the interrogation of tissue architecture and cell morphology by micro-CT.

Immunology and Infection

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

Shirley Albano-Aluquin¹, Jozef Malysz², Michal Kidacki³, Manohar Ratnam⁴, Nancy J. Olsen¹

¹Department of Medicine, Penn State University, ²Department of Pathology, Penn State University, ³Penn State College of Medicine, ⁴Department of Oncology, Wayne State University School of Medicine

The protocol illustrates the use of histopathologic examination and immunohistochemistry to profile the folate receptor beta macrophage and its relationship with the total immune cell infiltrate in temporal artery biopsies in giant cell arteritis.

Biology

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

Brian A. Hain¹, David L. Waning¹

¹Department of Cellular and Molecular Physiology, Penn State College of Medicine

Electroporation of plasmid DNA into skeletal muscle is a viable method to modulate gene expression without compromising muscle contractility in mice.