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Washington University School of Medicine, St. Louis, Missouri

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
Zuzana Kocsisova 1,2, Ariz Mohammad 2, Kerry Kornfeld 1, Tim Schedl 2
1Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

An imaging-based method is described that can be used to identify S-phase and analyze cell cycle dynamics in the C. elegans hermaphrodite germline using the thymidine analog EdU. This method requires no transgenes and is compatible with immunofluorescent staining.

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