Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

1 ARTICLES PUBLISHED IN JoVE

Environment

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

Tatsuo Ito¹, Keiki Ogino¹, Kenjiro Nagaoka¹, Kei Takemoto¹, Rina Nishiyama¹, Yurika Shimizu²

¹Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, ²Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

We present a method to detect 3-nitrotyrosine chemical modifications of atmospheric proteins with 6 mm-diameter rounds cut from air sampler prefilters using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD).