Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy CattleHeidi A. Rossow 1, Gabriela Acetoze 2, John Champagne 1, Jon J. Ramsey 3
1Veterinary Medicine Teaching and Research Center, University of California Davis, 2Archer Daniels Midland, 3Department of Molecular Biosciences, University of California Davis
Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.
