Second Hospital of Shanxi Medical University

6 ARTICLES PUBLISHED IN JoVE

Engineering

Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices

Yan Wu¹, Xiang Qian¹, Shengli Mi¹, Min Zhang¹, Shuqing Sun¹, Xiaohao Wang^1,2

¹Graduate School at Shenzhen, Tsinghua University, ²The State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University

Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.

Neuroscience

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Dandan Li^*1,2, Hóngyi Zhào^*2,3, Wei Wei², Nan Liu², Yonghua Dr. Huang²

¹Department of Neurology, Second Hospital of Shanxi Medical University, ²Department of Neurology, PLA Army General Hospital, ³Department of Neurology, NO 261 Hospital of PLA

We present a protocol to induce and detect CMHs caused by LPS injection in Sprague-Dawley rats, which may be utilized in future research investigations on the pathogenesis of CMHs.

Behavior

Individualized rTMS Treatment for Depression using an fMRI-Based Targeting Method

Xin Luo¹, Yiru Hu¹, Runhua Wang¹, Min Zhang¹, Xiaomei Zhong¹, Bin Zhang¹

¹Affiliated Brain Hospital of Guangzhou Medical University

The present protocol describes the application of repetitive transcranial magnetic stimulation (rTMS), where a subregion of the dorsolateral prefrontal cortex (DLPFC) with the strongest functional anticorrelation with the subgenual anterior cingulate cortex (sgACC) was located as the stimulation target under the assistance of a fMRI-based neuronavigation system.

Biology

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Qiang Yan^*1, Yingying He^*2,3, Yang Yue^2,3, Luyao Jie¹, Tingmei Wen^2,3, Yumiao Zhao^2,3, Min Zhang², Tingting Zhang²

¹State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, ²Research Institute of Applied Biology, Shanxi University, ³School of Life Science, Shanxi University

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

Chemistry

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Chunli Song^*1, Zhanfeng Hou^*1,2, Zijun Jiao^*1, Zhaodi Liu³, Chenshan Lian^1,2, Min Zhang¹, Wei Liang³, Feng Yin^1,2, Zigang Li^1,2

¹Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, ²Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, ³Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University

This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

Medicine

An Effective Surgical Strategy for Anteromedial Osteoarthritis of the Knee with Simultaneous Popliteal Cysts Drainage

Haohao Wang¹, Jiaju Yang¹, Yuhua Feng¹, Gang Xi¹, Min Zhang¹

¹Department of Orthopaedic Surgery, Second Hospital of Shanxi Medical University

Popliteal cysts are frequently found during the pre-operative examination of patients with osteoarthritis being prepared for the unicompartmental knee arthroplasty. These symptomatic cysts usually require treatment. To do this, a unicompartmental knee arthroplasty was performed with internal drainage of the popliteal cyst under the same anesthetic.