University of Ferrara and National Institute of Neuroscience

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Personalized Needles for Microinjections in the Rodent Brain

Giovanna Paolone^*1, Chiara Falcicchia^*1, Gianluca Verlengia^*1, Mario Barbieri¹, Anna Binaschi¹, Federico Paliotto¹, Beatrice Paradiso¹, Marie Soukupova¹, Silvia Zucchini^1,2, Michele Simonato^1,2

¹Department of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara, ²Laboratory for the Technologies for Advanced Therapies (LTTA), University of Ferrara

We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.

Neuroscience

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

Marie Soukupová¹, Chiara Falcicchia¹, Francesca Lovisari¹, Selene Ingusci¹, Mario Barbieri¹, Silvia Zucchini¹, Michele Simonato¹

¹Department of Medical Sciences, Section of Pharmacology, Neuroscience Center, University of Ferrara and National Institute of Neuroscience

Here, we describe a method for in vivo microdialysis to analyze aspartate and glutamate release in the ventral hippocampus of epileptic and non-epileptic rats, in combination with EEG recordings. Extracellular concentrations of aspartate and glutamate may be correlated with the different phases of the disease.