Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Spain

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Teresa Cordero¹, Verónica Aragonés¹, José-Antonio Daròs¹

¹Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Spain

Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to homogeneity.