Max Planck Institute for the Science of Light (MPL)

1 ARTICLES PUBLISHED IN JoVE

Engineering

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

André Gemeinhardt¹, Matthew P. McDonald^1,2, Katharina König^1,3, Michael Aigner⁴, Andreas Mackensen⁴, Vahid Sandoghdar^1,3

¹Max Planck Institute for the Science of Light (MPL), ²Area of Scientific Learning, Milligan College, ³Department of Physics, Friedrich Alexander University Erlangen-Nuremberg, ⁴Department of Internal Medicine 5, Hematology and Oncology, Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.