Université Grenoble Alpes, INRA, CNRS, CEA

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Imen Bouchnak¹, Lucas Moyet¹, Daniel Salvi¹, Marcel Kuntz¹, Norbert Rolland¹

¹Université Grenoble Alpes, INRA, CNRS, CEA

Here, we describe a method to purify intact chloroplasts from Arabidopsis leaves and their three main sub-compartments (envelope, stroma, and thylakoids), using a combination of differential centrifugations, continuous Percoll gradients, and discontinuous sucrose gradients. The method is valuable for subplastidial and subcellular localization of proteins by immunoblotting and proteomics.