Centre Hospitalier Universitaire des Alpes

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Magali Court^1,2, Marie Malier^1,2, Arnaud Millet^1,2,3

¹Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, ²Université Grenoble Alpes, ³Pôle Recherche, Centre Hospitalier Universitaire des Alpes

We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.