JoVE Logo

Sign In

Sorbonne Université, Paris, France

2 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
Fanny Etienne 1,2,3, Vincenzo Mastrolia 1,2,3, Luc Maroteaux 1,2,3, Jean-Antoine Girault 1,2,3, Nicolas Gervasi *1,2,3, Anne Roumier *1,2,3
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France

Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.

image

Biology

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
Hugo Lachuer 1,2,3, Pallavi Mathur 1,2,3, Kevin Bleakley 4, Kristine Schauer 1,2,3
1Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group, Institut Curie, 75005 Paris, France, 2PSL Research University, Paris, France, 3Sorbonne Université, Paris, France, 4INRIA, Université Paris-Sud, PSL

Live imaging of lysosomal exocytosis on micropatterned cells allows a spatial quantification of this process. Morphology normalization using micropatterns is an outstanding tool to uncover general rules about the spatial distribution of cellular processes.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved