Sorbonne Université, Paris, France

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

Fanny Etienne^1,2,3, Vincenzo Mastrolia^1,2,3, Luc Maroteaux^1,2,3, Jean-Antoine Girault^1,2,3, Nicolas Gervasi^*1,2,3, Anne Roumier^*1,2,3

¹Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, ²Sorbonne Université, Paris, France, ³Institut du Fer à Moulin, Paris, France

Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.

Biology

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Hugo Lachuer^1,2,3, Pallavi Mathur^1,2,3, Kevin Bleakley⁴, Kristine Schauer^1,2,3

¹Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group, Institut Curie, 75005 Paris, France, ²PSL Research University, Paris, France, ³Sorbonne Université, Paris, France, ⁴INRIA, Université Paris-Sud, PSL

Live imaging of lysosomal exocytosis on micropatterned cells allows a spatial quantification of this process. Morphology normalization using micropatterns is an outstanding tool to uncover general rules about the spatial distribution of cellular processes.