High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux AssayRoque Pastor-Ibáñez 1, Mireia Arnedo 1, Olivia Tort 1
1Group of Genomics and Pharmacogenomics in HIV, Laboratory of Retrovirology and Viral Immunopathogenesis, IDIBAPS, Hospital Clínic de Barcelona
Measurement of in vitro cholesterol efflux capacity to serum or plasma in macrophage cell models is a promising tool as a biomarker for atherosclerosis. In the present study, we optimize and standardize a fluorescent NBD-cholesterol efflux method and develop a high-throughput analysis using 96-well plates.
