Production, Purification, and Quality Control for Adeno-associated Virus-based VectorsShelly Fripont *1,2, Catherine Marneffe *1,2, Marika Marino *1,2, Melvin Y. Rincon 1,2, Matthew G. Holt 1,2,3
1VIB-KU Leuven Center for Brain and Disease Research, 2Department of Neuroscience, KU Leuven, 3Leuven Brain Institute
Here, we describe an efficient and reproducible strategy to produce, titer, and quality-control batches of adeno-associated virus vectors. It allows the user to obtain a vector preparation with high-titer (≥1 x 1013 vector genomes/mL) and a high purity, ready for in vitro or in vivo use.
