Combined In Vivo Electroporation and Short-Term Reinnervation of the Cranial Levator Auris Longus Skeletal MuscleJessica Mella *1, Francisca Bermedo-Garcia *1, Angelymar Medina-Moreno 1, Jorge Ojeda 2, Juan Pablo Henríquez 1,3
1Neuromuscular Studies Lab (NeSt Lab), Institute of Anatomy, Histology, and Pathology, Faculty of Medicine, Universidad Austral de Chile, 2Faculty of Odontology and Rehabilitation Sciences, Universidad San Sebastian, 3Department of Cell Biology, Faculty of Biological Sciences, Universidad de Concepcion
Here, we present a protocol that combines in vivo electroporation and denervation of the cranial levator auris longus (LAL) muscle. This procedure enables the study of the potential role of muscle-derived proteins in the regeneration of the neuromuscular synapse.