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Florida State University College of Medicine

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Extraction of Extracellular Vesicles from Whole Tissue
Stephanie N. Hurwitz 1, James M. Olcese 1, David G. Meckes Jr. 1
1Department of Biomedical Sciences, Florida State University College of Medicine

Here, we provide a detailed protocol to isolate small extracellular vesicles (EVs) from whole tissues, including brain and tumor specimens. This method offers a reproducible technique to extract EVs from solid tissue for further downstream analyses.

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Biology

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
Ernest O. N. Phillips *1, Serena Giovinazzi *1, Sarah L. Menz *1, Yuna Son 1, Akash Gunjan 1
1Department of Biomedical Sciences, Florida State University College of Medicine

We describe a reliable method for the preparation of whole cell extracts from yeast or other cells using a cryogenic freezer mill that minimizes degradation and denaturation of proteins. The cell extracts are suitable for purification of functional protein complexes, proteomic analyses, co-immunoprecipitation studies and detection of labile protein modifications.

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