Florida State University College of Medicine

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Extraction of Extracellular Vesicles from Whole Tissue

Stephanie N. Hurwitz¹, James M. Olcese¹, David G. Meckes Jr.¹

¹Department of Biomedical Sciences, Florida State University College of Medicine

Here, we provide a detailed protocol to isolate small extracellular vesicles (EVs) from whole tissues, including brain and tumor specimens. This method offers a reproducible technique to extract EVs from solid tissue for further downstream analyses.

Biology

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

Ernest O. N. Phillips^*1, Serena Giovinazzi^*1, Sarah L. Menz^*1, Yuna Son¹, Akash Gunjan¹

¹Department of Biomedical Sciences, Florida State University College of Medicine

We describe a reliable method for the preparation of whole cell extracts from yeast or other cells using a cryogenic freezer mill that minimizes degradation and denaturation of proteins. The cell extracts are suitable for purification of functional protein complexes, proteomic analyses, co-immunoprecipitation studies and detection of labile protein modifications.