Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

1 ARTICLES PUBLISHED IN JoVE

Biology

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Anna M. Steyer^1,2, Torben Ruhwedel¹, Wiebke Möbius^1,2

¹Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine, ²Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Here we present a protocol for combining two sample processing techniques, high-pressure freezing and microwave-assisted sample processing, followed by minimal resin embedding for acquiring data with a focused ion beam scanning electron microscope (FIB-SEM). This is demonstrated using a mouse tibial nerve sample and Caenorhabditis elegans.