Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

Anaelle da Costa^*1, Christophe Prehaud^*1, Florian Bakoa¹, Philippe Afonso², Pierre-Emmanuel Ceccaldi², Pierre Lafaye³, Monique Lafon¹

¹Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, ²Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, ³Institut Pasteur, PFIA, DTPS

Here we present a protocol describing the setting of an in cellulo BBB (Blood brain barrier)-Minibrain polyester porous membrane culture insert system in order to evaluate the transport of biomolecules or infectious agents across a human BBB and their physiological impact on the neighboring brain cells.