Prinses Máxima Center for Pediatric Oncology

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy

¹Cancer Genomics Netherlands, Prinses Máxima Center for Pediatric Oncology

Here we describe a method for high-resolution time-lapse multiphoton imaging of brain tumor cells before and after invasive surgical intervention (e.g., biopsy) within the same living animal. This method allows studying the impact of these invasive surgical procedures on tumor cells' migratory, invasive, and proliferative behavior at a single cell level.

Biology

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Ravian L. van Ineveld^*1,2,3, Hendrikus C.R. Ariese^*1,2,3, Ellen J. Wehrens^1,2,3, Johanna F. Dekkers^*1,2,3,4, Anne C. Rios^*1,2,3

¹Princess Máxima Center for Pediatric Oncology, ²Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, ³Cancer Genomics Center (CGC), ⁴Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.