Sapienza University of Rome, Italy

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Maria G. Garone¹, Valeria de Turris², Alessandro Soloperto², Carlo Brighi², Riccardo De Santis³, Francesca Pagani^2,4, Silvia Di Angelantonio^2,4, Alessandro Rosa^1,2

¹Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, ²Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, ³Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, ⁴Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

This protocol allows rapid and efficient conversion of induced pluripotent stem cells into motor neurons with a spinal or cranial identity, by ectopic expression of transcription factors from inducible piggyBac vectors.