Università del Piemonte Orientale

2 ARTICLES PUBLISHED IN JoVE

Medicine

Manufacture of a Multi-Purpose Low-Cost Animal Bench-Model for Teaching Tracheostomy

Federico Sacco Botto¹, Pier Luigi Ingrassia², Paolo Donato³, Massimiliano Garzaro⁴, Paolo Aluffi⁴, Sergio Gentilli⁵, Massimo Olina⁶, Elena Grossini¹

¹Physiology and Experimental Surgery, Department of Translational Medicine, Università del Piemonte Orientale, ²Centro Interdipartimentale di Didattica Innovativa e di Simulazione in Medicina e Professioni Sanitarie - SIMNOVA, Università del Piemonte Orientale, ³Section of Anesthesia and Intensive Care, Azienda Ospedaliera Maggiore della Carità, Università del Piemonte Orientale, ⁴Section of ENT, Azienda Ospedaliera Maggiore della Carità, Department of Health Sciences, Università del Piemonte Orientale, ⁵Section of General Surgery Department of Health of Sciences, Azienda Ospedaliera Maggiore della Carità, Università del Piemonte Orientale, ⁶Department of Education, Azienda Ospedaliera Maggiore della Carità

This article illustrates every step of the manufacture of a new multi-purpose low-cost animal bench-model for subglottic airway access management. All the procedures are shown in the video. The model's realism and its suitability for training the given clinical maneuvers were assessed by independent senior otolaryngologists and anesthesiologists.

Biochemistry

Optimized Methods for the Surface Immobilization of Collagens and Collagen Binding Assays

Nadia Chaher¹, Giuseppe Digilio^*2, Sara Lacerda^*3, René M. Botnar^1,4,5,6, Alkystis Phinikaridou^1,4

¹School of Biomedical Engineering and Imaging Sciences, King’s College London, ²Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, ³Centre de Biophysique Moléculaire, CNRS UPR 4301, Université d'Orléans, ⁴BHF Centre of Research Excellence, Cardiovascular Division, King’s College London, ⁵Escuela de Ingeniería, Pontificia Universidad Católica de Chile, ⁶Instituto de Ingeniería Biológica y Médica, Pontificia Universidad Católica de Chile

This work presents an optimized protocol to reproducibly immobilize and quantify type I and III collagen onto microplates, followed by an improved in vitro binding assay protocol to study collagen-compound interactions using a time-resolved fluorescence method. The subsequent step-by-step data analysis and data interpretation are provided.