Singapore-MIT Alliance for Research and Technology, CREATE

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Chia-Hua Lee¹, Suman Bose², Krystyn J. Van Vliet¹, Jeffrey M. Karp³, Rohit Karnik²

¹Department of Materials Science and Engineering, MIT - Massachusetts Institute of Technology, ²Department of Mechanical Engineering, MIT - Massachusetts Institute of Technology, ³HST Center for Biomedical Engineering and Harvard Stem Cell Institute, Brigham and Women's Hospital and Harvard Medical School

We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.

Neuroscience

Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry

Elizabeth Peruski Canovic¹, Bo Qing², Aleksandar S. Mijailovic³, Anna Jagielska², Matthew J. Whitfield², Elyza Kelly⁴, Daria Turner⁴, Mustafa Sahin⁴, Krystyn J. Van Vliet^1,2

¹Department of Materials Science and Engineering, Massachusetts Institute of Technology, ²Department of Biological Engineering, Massachusetts Institute of Technology, ³Department of Mechanical Engineering, Massachusetts Institute of Technology, ⁴Department of Neurology, The F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School

We present a set of techniques to characterize the viscoelastic mechanical properties of brain at the micro-, meso-, and macro-scales.

Bioengineering

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Ekta Makhija¹, Anna Jagielska^1,2, Krystyn J. Van Vliet^1,2,3

¹BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, CREATE, ²Department of Material Science and Engineering, Massachusetts Institute of Technology, ³Department of Biological Engineering, Massachusetts Institute of Technology

Using a previously designed device to apply mechanical strain to adherent cells, this paper describes a redesigned substratum geometry and a customized apparatus for high-resolution single-cell imaging of strained cells with a 100x oil immersion objective.