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National Research Council of Italy (CNR)

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Methods to Test Endocrine Disruption in Drosophila melanogaster
Tiziana Francesca Bovier 1, Daniela Cavaliere 1, Michele Colombo 1, Gianfranco Peluso 2, Ennio Giordano 3, Filomena Anna Digilio 1,2
1Institute of Biosciences and BioResources, National Research Council of Italy (CNR), 2Institute of Research on Terrestrial Ecosystems (IRET), National Research Council of Italy (CNR), 3Department of Biology, UniNa "Federico II"

Endocrine disruptor chemicals (EDCs) represent a serious problem for organisms and for natural environments. Drosophila melanogaster represents an ideal model to study EDC effects in vivo. Here, we present methods to investigate endocrine disruption in Drosophila, addressing EDC effects on fecundity, fertility, developmental timing, and lifespan of the fly.

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Immunology and Infection

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
Sonia Simonetti *1,2, Ambra Natalini *1,2, Giovanna Peruzzi 3, Alfredo Nicosia 4, Antonella Folgori 5, Stefania Capone 5, Angela Santoni 2,6, Francesca Di Rosa 1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated mice.

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