A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
7.1K Views
•
09:17 min
•
January 5th, 2021
DOI :
January 5th, 2021
•Sonia Simonetti*1,2, Ambra Natalini*1,2, Giovanna Peruzzi3, Alfredo Nicosia4, Antonella Folgori5, Stefania Capone5, Angela Santoni2,6, Francesca Di Rosa1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
Chapters in this video
0:04
Introduction
0:57
Intracellular Staining
2:41
Data Analysis
7:16
Results: Cell Cycle Analysis of BM Cells and Antigen-Specific CD8 T Cells form LNs and Spleen
8:30
Conclusion
Related Videos
10:14
Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
17.9K Views
12:34
Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
7.4K Views
11:07
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
13.2K Views
12:19
Co-Culture In Vitro Systems to Reproduce the Cancer-Immunity Cycle
784 Views
08:10
Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
8.2K Views
09:03
Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
8.6K Views
06:07
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
6.5K Views
06:42
Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells
1.2K Views
10:57
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
24.2K Views
01:28
An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells
86 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved