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Hôpital du Sacré-Cœur de Montréal

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Neurodegeneration in an Animal Model of Chronic Amyloid-beta Oligomer Infusion Is Counteracted by Antibody Treatment Infused with Osmotic Pumps
Ahmadali Sajadi 1,3, Chloé Provost 3, Brendon Pham 2, Jonathan Brouillette 1,3
1Department of Pharmacology, Université de Montréal, 2Department of Neuroscience, Université de Montréal, 3Hôpital du Sacré-coeur de Montréal Research Center, Université de Montréal

To study the effects of Aβo in vivo, we developed a model based on repeated hippocampal infusions of soluble Aβo coupled with continuous infusion of Aβo antibody (6E10) in the hippocampus using osmotic pumps to counteract the neurotoxic effect of Aβo.

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Neuroscience

A Novel and Translational Rat Model of Concussion Combining Force and Rotation with In Vivo Cerebral Microdialysis
Ian O. Massé 1, Luc Moquin 2, Chloé Provost 1, Samuel Guay 1, Alain Gratton 2, Louis De Beaumont 1
1Research Center, Hôpital du Sacré-Cœur de Montréal, 2Research Center, Douglas Institute

Neurotransmitter alteration is a mechanism of neural dysfunction that occurs after concussion and contributes to the sometimes-catastrophic long-term consequences. This rat model combines microdialysis, allowing in vivo neurotransmitter quantification, with a weight-drop technique exerting rapid acceleration and deceleration of the head and torso, an important factor of human craniocerebral trauma.

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Neuroscience

Thoracic Spinal Cord Hemisection Surgery and Open-Field Locomotor Assessment in the Rat
Andrew R. Brown 1,2, Marina Martinez 1,2,3
1Department of Neurosciences, Faculté de Médecine, Université de Montréal, 2Hôpital du Sacré-Cœur de Montréal, 3Groupe de Recherche sur le Système Nerveux Central (GRSNC), Université de Montréal

The rat thoracic spinal hemisection is a valuable and reproducible model of unilateral spinal cord injury to investigate the neural mechanisms of locomotor recovery and treatment efficacy. This article includes a detailed step-by-step guide to perform the hemisection procedure and to assess locomotor performance in an open-field arena.

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