Quebec Network for Research on Protein Function, Structure, and Engineering

1 ARTICLES PUBLISHED IN JoVE

Genetics

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Marie A. Brunet^1,2, Xavier Roucou^1,2

¹Department of Biochemistry, Université de Sherbrooke, ²PROTEO, Quebec Network for Research on Protein Function, Structure, and Engineering

OpenProt is a freely accessible database that enforces a polycistronic model of eukaryotic genomes. Here, we present a protocol for the use of OpenProt databases when interrogating mass spectrometry datasets. Using OpenProt database for analysis of proteomic experiments allows for discovery of novel and previously undetectable proteins.