National Research Council, Institute for Microelectronics and Microsystems, Naples Unit, Italy

1 ARTICLES PUBLISHED IN JoVE

Engineering

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Rajeev Ranjan¹, Maurizio Indolfi¹, Maria Antonietta Ferrara¹, Luigi Sirleto¹

¹National Research Council, Institute for Microelectronics and Microsystems, Naples Unit, Italy

In this manuscript, the implementation of a stimulated Raman scattering (SRS) microscope, obtained by the integration of an SRS experimental set-up with a laser scanning microscope, is described. The SRS microscope is based on two femtosecond (fs) laser sources, a Ti-Sapphire (Ti:Sa) and synchronized optical parametric oscillator (OPO).