University of Huddersfield

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

Jane Harmer¹, Nina Struve², Anke Brüning-Richardson¹

¹School of Applied Sciences, University of Huddersfield, ²Laboratory of Radiotherapy and Experimental Radiation Oncology, Humbertus Wald Tumorzentrum, University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf

The effects of migrastatic inhibitors on glioma cancer cell migration in three-dimensional (3D) invasion assays using a histone deacetylase (HDAC) inhibitor are characterized by high-resolution confocal microscopy.

Bioengineering

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

Jessica J. Senior^*1, Richard J. A. Moakes^*2, Megan E. Cooke³, Samuel R. Moxon⁴, Alan M. Smith¹, Liam M. Grover²

¹Department of Pharmacy, University of Huddersfield, ²School of Chemical Engineering, University of Birmingham, ³Chemical and Biological Engineering, University of Colorado Boulder, ⁴School of Biological Sciences, University of Manchester

Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described.