School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Ardjan J. van der Linden¹, Maaruthy Yelleswarapu², Pascal A. Pieters¹, Zoe Swank³, Wilhelm T. S. Huck², Sebastian J. Maerkl³, Tom F. A. de Greef^1,2

¹Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, ²Institute for Molecules and Materials, Radboud University, ³Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)

The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.

Bioengineering

OnePot PURE Cell-Free System

Laura Grasemann^*1, Barbora Lavickova^*1, M. Carolina Elizondo-Cantú¹, Sebastian J. Maerkl¹

¹Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne

We present a fast and cost-effective method to produce the recombinant PURE cell-free TX-TL system using standard laboratory equipment.