JoVE Logo
Faculty Resource Center

Sign In

OnePot PURE Cell-Free System

DOI :

10.3791/62625-v

8:25 min

June 23rd, 2021

June 23rd, 2021

6,671 Views

1Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne

We present a fast and cost-effective method to produce the recombinant PURE cell-free TX-TL system using standard laboratory equipment.

Tags

AI Writing Assistant

-- Views

Related Videos

article

The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics

article

Sealable Femtoliter Chamber Arrays for Cell-free Biology

article

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

article

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens

article

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

article

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

article

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

article

Rapid Characterization of Genetic Parts with Cell-Free Systems

article

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

article

From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved