A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

10.1K Views

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11:23 min

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October 6th, 2019

DOI :

10.3791/59655-v

October 6th, 2019

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Ardjan J. van der Linden¹, Maaruthy Yelleswarapu², Pascal A. Pieters¹, Zoe Swank³, Wilhelm T. S. Huck², Sebastian J. Maerkl³, Tom F. A. de Greef¹^,²

¹Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, ²Institute for Molecules and Materials, Radboud University, ³Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)

Summary

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Multilayer Microfluidic Platform

Cell free Gene Expression

Synthetic Gene Networks

GFP Expression

Microfluidic Flow Reactors

Biochemical Reactions

Cooling System

PTFE Tubing

Peltier Element

Temperature Controller

Thermistor

Flow Pressure Regulation

Experimental Platform Fabrication

Rapid Prototyping

Chapters in this video

0:04

Title

1:00

Off-Chip Cooling Setup

2:35

Preparing the Setup for an Experiment

4:20

Connecting Uncooled Reagents to the Device

5:45

Connecting Cooled Fluids to the Microfluidic Device

6:49

Loading the Microfluidic Device

8:09

Calibrating the Microfluidic Device

8:56

Results: IVTT Experiment Expressing the deGFP Protein

10:40

Conclusion

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