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Showa University School of Pharmacy

1 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Takashi Obama *1, Takuro Miyazaki *2, Toshihiro Aiuchi 1, Akira Miyazaki 2, Hiroyuki Itabe 1
1Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 2Department of Biochemistry, Showa University School of Medicine

Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.

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