JoVE Logo

Sign In

Showa University School of Pharmacy

1 ARTICLES PUBLISHED IN JoVE

image

JoVE Journal

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Takashi Obama *1, Takuro Miyazaki *2, Toshihiro Aiuchi 1, Akira Miyazaki 2, Hiroyuki Itabe 1
1Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 2Department of Biochemistry, Showa University School of Medicine

Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved