Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase ProteinBéatrice Vallée 1, Michel Doudeau 1, Fabienne Godin 1, Hélène Bénédetti 1
1Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM
We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling.
