In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid InteractionsLina Yu *1, Wenxiu He *1, Jie Xie *1, Rui Guo *1, Juan Ni 2, Xia Zhang 3, Quishi Xu 1, Caifeng Wang 1, Qiuling Yue 1, Fangfang Li 1, Mengcheng Luo 4, Bo Sun 3, Lan Ye 1, Ke Zheng 1
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology, ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University
Presented here are protocols for in vitro biochemical assays using biotin labels that may be widely applicable for studying protein-nucleic acid interactions.