JoVE Logo

Sign In

São Paulo State University

3 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Short-Term Free-Floating Slice Cultures from the Adult Human Brain
Artur Fernandes 1,2, Niele Dias Mendes 1,3, Glaucia Maria Almeida 1, Giovanna Orlovski Nogueira 1, Carla de Moraes Machado 4, Jose de Anchieta de Castro Horta-Junior 4, João Alberto Assirati Junior 5, Norberto Garcia-Cairasco 2, Luciano Neder 3, Adriano Sebollela 1
1Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, 2Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, 3Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, 4Department of Anatomy, Institute of Biosciences, São Paulo State University, 5Clinical Hospital at the Ribeirão Preto Medical School, University of São Paulo

A protocol to prepare free-floating slice cultures from adult human brain is presented. The protocol is a variation of the widely used slice culture method using membrane inserts. It is simple, cost-effective, and recommended for running short-term assays aimed to unravel mechanisms of neurodegeneration behind age-associated brain diseases.

image

Developmental Biology

Differentiation of Porcine Induced Pluripotent Stem Cells (piPSCs) into Neural Progenitor Cells (NPCs)
Lucas Simões Machado 1, Kaiana Recchia 1,2, Naira Caroline Godoy Pieri 2, Ramon Cesar Botigelli 2,3, Raquel Vasconcelos Guimarães de Castro 2, Jéssica Brunhara Cruz 1, Laís Vicari de Figueiredo Pessôa 2, Fabiana Fernandes Bressan 1,2
1Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, 2Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, 3Department of Pharmacology, Institute of Biosciences, São Paulo State University

This protocol describes a method for chemical differentiation and culture of neural progenitor cells derived from porcine induced pluripotent stem cells (piPSCs).

image

Biology

Collection and Long-Term Maintenance of Leaf-Cutting Ants (Atta) in Laboratory Conditions
Bianca Raissa Nogueira 1, Amanda Aparecida de Oliveira 1, Janiele Pereira da Silva 2, Odair Correa Bueno 1
1Institute of Biosciences, Department of General and Applied Biology, Laboratory of Leaf-cutting Ants, São Paulo State University, 2Institute of Psychology, Laboratory Walter Hugo Cunha, São Paulo University

Here, a protocol is described to successfully collect and maintain healthy Atta (Hymenoptera: Formicidae) ant colonies in laboratory conditions. Additionally, different nest types and configurations are detailed together with possible experimental procedures.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved