Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

Xiangzhen Yan^*1, Xing Ran^*2, Siying Xia¹, Yanan Yang¹, Min Zhou¹, Chunxue Yuan³, Lijun Luo¹

¹Department of Periodontology, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, ²Department of Periodontology, Dental Diseases Prevention & Treatment Center of Jiading District, ³College of Materials Science and Engineering, Tongji University

Here, we present protocols of culturing human periodontal ligament (PDL) cell spheroids by chitosan films. The culture of three-dimensional (3D) cellular spheroids provides an alternative to conventional tissue culture polystyrene (TCPS) culture system.