Toxicology Program, ILS, Inc.

1 ARTICLES PUBLISHED IN JoVE

Genetics

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Cheryl A. Hobbs^*1, Leslie Recio¹, John Winters¹, Kristine L. Witt²

¹Toxicology Program, ILS, Inc., ²Division of the National Toxicology Program, National Institute of Environmental Health Sciences

This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.