Identifying Mutations by High Resolution Melting in a TILLING Population of RiceShan Li 1, Ying-Ping Yu 1, Song-Mei Liu 1, Hao-Wei Fu 2, Jian-Zhong Huang 3, Qing-Yao Shu 1, Yuan-Yuan Tan 4
1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences, Zhejiang University, 4The New Countryside Development Institute at Zhejiang University
In this article, we present the protocol that is described as high-resolution melting analysis (HRM)-based Target Induced Local Lesions In Genomes (TILLING). This method utilizes fluorescence changes during the melting of the DNA duplex and is suitable for high-throughput screening of both insertion/deletion (Indel) and single base substition (SBS).
