Henry M. Jackson Foundation for the Advancement of Military Medicine

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Christopher P. Karch^1,2, Peter Burkhard³, Gary R. Matyas¹, Zoltan Beck^1,2

¹U.S. Military HIV Research Program, Walter Reed Army Institute of Research, ²Henry M. Jackson Foundation for the Advancement of Military Medicine, ³Alpha-O Peptides AG

A detailed method is provided here describing the purification, refolding, and characterization of self-assembling protein nanoparticles (SAPNs) for use in vaccine development.