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Charité - University Medicine

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
Anton Dvorzhak 1, Rosemarie Grantyn 1
1Synaptic Dysfunction Group, Neuroscience Research Center, Charité - University Medicine

We present a protocol to evaluate the balance between glutamate release and clearance at single corticostriatal glutamatergic synapses in acute slices from adult mice. This protocol uses the fluorescent sensor iGluu for glutamate detection, a sCMOS camera for signal acquisition and a device for focal laser illumination.

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