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The Ohio State University Comprehensive Cancer Center

3 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
Elliott J. Hagedorn 1,2, Jennifer L. Cillis 3, Caitlyn R. Curley 3, Taylor C. Patch 3, Brian Li 1,2, Bradley W. Blaser 1,2,7, Raquel Riquelme 1,2, Leonard I. Zon 1,2,4,5,6, Dhvanit I. Shah 1,2,3,4,5
1Division of Hematology/Oncology, Boston Children’s Hospital, 2Harvard Medical School, 3Division of Hematology, Department of Medicine, Brigham and Women’s Hospital, 4Harvard Stem Cell Institute, 5Broad Institute of Massachusetts Institute of Technology, 6Howard Hughes Medical Institute, 7Division of Hematologic Malignancies, Dana-Farber Cancer Institute

This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.

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JoVE Journal

Analysis of Hematopoietic Stem Progenitor Cell Metabolism
Giorgia Scapin 1,2,3, Marie C. Goulard 1,2,3, Priyanka R. Dharampuriya 1,2,3, Jennifer L. Cillis 1,2,3, Dhvanit I. Shah 1,2,3
1Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

Hematopoietic stem progenitor cells (HSPCs) transition from a quiescent state to a differentiation state due to their metabolic plasticity during blood formation. Here, we present an optimized method for measuring mitochondrial respiration and glycolysis of HSPCs.

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Medicine

Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography
Qiang Zeng 1, Doug A. Gouchoe 2,3, Mahboubeh Nabavinia 4, Yong Gyu Lee 2, Xi Wang 2, Terri A. Shaffer 5, Mitchel R. Stacy 4,5,6, Blake R. Peterson 7,8, Bryan A. Whitson 2,9, Christopher Breuer 1,4, Sylvester M. Black 1,10
1Department of Surgery, Nationwide Children’s Hospital, 2COPPER Laboratory, The Ohio State University, 388th Surgical Operation Operations Squadron, WPAFB, 4Center for Regenerative Medicine, The Research Institute at Nationwide Children’s Hospital, 5Division of Vascular Diseases and Surgery, The Ohio State University College of Medicine, 6Interdisciplinary Biophysics Graduate Program, The Ohio State University, 7Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University College of Pharmacy, 8The Ohio State University Comprehensive Cancer Center, 9Division of Cardiac Surgery, Department of Surgery, The Ohio State Wexner Medical Center, 10Division of Transplantation, Department of Surgery, The Ohio State Wexner Medical Center

In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.

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