Biogen, Inc.

5 ARTICLES PUBLISHED IN JoVE

Medicine

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Lei Li^*1, Xiaoyun Guo^*1, Yi Chen¹, Haifeng Yin¹, Jing Li¹, Jessica Doan¹, Qinghang Liu¹

¹Department of Physiology and Biophysics, University of Washington

The goal of this protocol is to noninvasively assess cardiac structural and functional changes in a mouse model of heart disease created by transverse aortic constriction, using B- and M-mode echocardiography and color/pulse wave Doppler imaging.

Bioengineering

Microhoneycomb Monoliths Prepared by the Unidirectional Freeze-drying of Cellulose Nanofiber Based Sols: Method and Extensions

Zheng-Ze Pan^1,2, Hirotomo Nishihara³, Wei Lv¹, Cong Wang^1,2, Yi Luo^1,2, Liubing Dong^1,2, Houfu Song^1,4, Wenjie Zhang², Feiyu Kang^1,2,4, Takashi Kyotani³, Quan-Hong Yang^1,4,5

¹Engineering Laboratory for Functionalized Carbon Materials and Shenzhen Key Laboratory for Graphene-based Materials, Graduate School at Shenzhen, Tsinghua University, ²School of Materials Science and Engineering, Tsinghua University, ³Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, ⁴Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, ⁵School of Chemical Engineering and Technology, Tianjin University

Here, we present a general protocol to prepare a variety of microhoneycomb monoliths (MHMs) in which fluid can pass through with an extremely low pressure drop. MHMs obtained are expected to be used as filters, catalyst supports, flow-type electrodes, sensors and scaffolds for biomaterials.

Neuroscience

Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System

Yi Chen^*1, Curt Mazur^*2, Yi Luo^*1, Linhong Sun¹, Mingdi Zhang¹, Alex McCampbell¹, Giulio Srubek Tomassy¹

¹Biogen, Inc., ²Ionis Pharmaceuticals

Here, we describe a method for delivering drugs to the rat central nervous system by implanting a catheter into the lumbar intrathecal space of the spine. We focus on the delivery of antisense oligonucleotides, though this method is suitable for delivery of other therapeutic modalities as well.

Bioengineering

Viscoelastic Characterization of Soft Tissue-Mimicking Gelatin Phantoms using Indentation and Magnetic Resonance Elastography

Yuan Feng¹, Suhao Qiu¹, Yu Chen¹, Runke Wang¹, Zhao He¹, Linghan Kong¹, Yi Chen¹, Shengyuan Ma¹

¹School of Biomedical Engineering, Shanghai Jiao Tong University

This article presents a demonstration and summary of protocols of making gelatin phantoms that mimic soft tissues, and the corresponding viscoelastic characterization using indentation and magnetic resonance elastography.

Medicine

The Miniature Pig: A Large Animal Model for Cochlear Implant Research

Xiaojun Ji^*1,2,3,4, Yi Luo^*1,2,3,4, Weiwei Guo^1,2,3,4, Fei Ji^1,2,3,4, Shuolong Yuan^1,2,3,4, Liangwei Xu^1,2,3,4, Wei Chen^*1,2,3,4

¹College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, ²National Clinical Research Center for Otolaryngologic Diseases, ³Key Lab of Hearing Science, Ministry of Education, ⁴Beijing Key Lab of Hearing Impairment for Prevention and Treatment

Miniature pigs (mini-pigs) are an ideal large animal model for research into cochlear implants. Cochlear implantation surgery in mini-pigs can be utilized to provide initial evidence of the safety and potential performance of novel electrode arrays and surgical approaches in a living system similar to human beings.